Canine and Feline Cytology: A Color Atlas and Interpretation Guide (2nd Edition)

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Harry Potter. Popular Features. New Releases. This easy-to-use guide covers all body systems and fluids including a special chapter on acquisition and management of cytology specimens. Hundreds of vivid color images of normal tissue alongside abnormal tissue images - plus concise summaries of individual lesions and guidelines for interpretation - will enhance your ability to confidently face any diagnostic challenge.

Product details Format Hardback pages Dimensions Meyer Sara L. Rebar Craig A. Andreasen Albert E. Jergens Dennis J. Raskin Chapter Endocrine System A. Ramos-Vara Anne C. Avery Paul R. Avery show more. Review quote "The book is full of colour illustrations, more than in fact, and is accompanied by a clear text. C, Butterfly needle. Using a butterfly needle attached to the syringe will allow more flexibility with fractious patients when removing fluid.

A three-way stopcock can be placed between the butterfly tubing and syringe to facilitate the removal of large amounts of fluid.

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  • Canine and Feline Cytology: A Color Atlas and Interpretation Guide, 3rd Edition!

D, Ultrasound guidance. Free-hand technique for ultrasound-guided fine-needle aspiration biopsy. E, Ultrasound guidance. Biopsy guide is attached to a linear transducer that holds a needle firmly for ultrasound-guided fine-needle aspiration biopsy. B, Courtesy of Delasco. Complications but is rare with gauge or smaller aspiration needles. Hypertension and Patients should be evaluated for bleeding disorders paradoxic hypotension has been reported in dogs follow- before FNAB, especially when highly vascular tissues are ing ultrasound-guided FNAB of pheochromocytoma of the sampled.

In humans, adrenal gland must be performed cautiously when pheochro- implantation is associated with the use of large-bore needles mocytoma is suspected. A, The application of only a small drop or a portion of the specimen on the glass slide near the frosted end is an important initial step for making a quality cytologic preparation. D, A squash preparation can be made by gently placing the top slide parallel to the bottom slide and gliding apart with even pressure.

Keep the flat surfaces of the two slides parallel. A common mistake able procedure for the management of cytology specimens that is to slightly angle the upper slide near the end of the gliding motion by are semisolid, mucus-like, or pelleted by centrifugation. A small allowing a slight counterclockwise rotation of the wrist clockwise if left amount of material is placed on a clean glass slide approxi- handed to occur, causing cell lysis or uneven spread of the specimen. A sec- scraping sound of glass on glass can be heard when this occurs.

Again, ond clean glass slide is placed over the specimen at right angles. The same concept applies to the and even stain penetration. The compression preparation thus location of the cytologic specimen if it is to make a successful diagnostic hit.

Canine and Feline Cytology: A Color Atlas and Interpretation Guide - PDF Drive

A properly prepared glass slide is characterized by erly examined. When slides go through an automated stainer, their guiding a feather-shaped oblong area, with a monolayer end referred tracks can scrape off diagnostic material that is too close to the end of the to as the sweet spot Fig. An alternate method for the slide Fig. Material placed too far from the end the specimen may not squash preparation is placing the top slide parallel to the bottom be exposed adequately to the stain. The ends and longitudinal edges of the slide Fig.

Sedimentation preparations can also be used to concentrate cells in bloody, cloudy, or wash fluids. The sample can be cen- trifuged in the same tube in which it was collected after direct preparations have been made. After centrifugation, the major- ity of supernatant is removed with a pipette and the cell pellet resuspended in the remaining fluid.

Canine and Feline Cytology: Color Atlas and Interpretation Guide, 2nd edition

It is important to remember that estimates of cell counts cannot be made on concentrated samples, only from the direct smear. Following these and other concentration techniques, cell blocks can be made from the pelleted material to be evaluated histopathologically and immunohistochemically.

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See Appen- dix for more information. The clear area to the left represents men is enhanced with the buffy-coat concentration technique.

Canine and Feline Cytology: A Color Atlas and Interpretation Guide, 3rd Edition

Wright; LP. A direct smear or squash technique is used to spread the spec- imen.

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It is also useful ably is. Make another one. If the cytology specimen appears to be too for the examination of peripheral blood for neoplastic cells and close to the end or edge of the slide, it probably is. If cell-associated infectious organisms. The use of a cytocentrifuge cytospin is recom- mended for the capture of all the cells Fig. For cere- brospinal fluid, a cytologic preparation should be made—ideally Management of Fluids within 30 to 60 minutes because the low specific gravity predis- A fluid specimen should be immediately placed in an EDTA poses to cellular lysis.

However, it appears that when inflamma- tube to prevent clot formation. Fluid with a plasma-like consis- tory and neoplastic cells and infectious agents are present, their tency can be handled in a fashion similar to the preparation of diagnostic cellular integrity is usually maintained for up to 12 a blood smear. The speed at rect, centrifuged or buffy coat , and cytospin if possible preparations and which the slide is moved depends on the viscosity of the sam- assess each for the best diagnostic yield.

It is tempting to go off the end of the slide with information has diagnostic importance Meyer and Harvey, The use of premade serum-coated slides facilitates the These cellular clumps often follow the spreader slide, finally adhesion of the cells, which can make a diagnostic world of difference. Ten to 20 slide preparations are made.

Once dry not sticky to the spreader slide to another clean glass slide, and repeat the the touch , the slides can be stacked together in an empty slide box and spreading procedure. When minimal excess fluid remains, the placed in the freezer to prevent bacterial growth.

Prior to use, several fluid can be permitted to slowly flow back on itself for a short slides are brought to room temperature.

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It is critical that no condensation distance. The thin part of the stained cytology slide preparation develops on the surface because it causes severe cell lysis. A, The procedure for making a cytologic preparation from a fluid specimen is illustrated. B, The spreader slide is slowly backed into the drop.

C, Just as the fluid begins to spread along its edge, the spreader slide is glided away from the frosted end. D, All of the original fluid drop should remain on the slide, and the temptation to go off the end of the slide with excess fluid must be avoided.

Canine and Feline Cytology

The lower slide illustrates a properly feathered fluid specimen with the entire specimen remaining on the slide. E, Excess fluid that remains is allowed to partially flow back and is air-dried as illustrated by the small opaque dried fluid triangle near the nonfrosted end of the slide. Alternatively, the edge of the spreader slide with the excess fluid adhering is transferred to another clean slide and another smear made.

A, Examination of the feathered edge of the lower slide pictured in Fig. The area to the right of the cell clumps consisted of only erythrocytes.